By Douglas E. Caldwell, Darren R. Korber, John R. Lawrence (auth.), K. C. Marshall (eds.)
Advances in Microbial Ecology used to be verified through the overseas Committee on Microbial Ecology (ICOME) to supply a motor vehicle for in-depth, severe, or even provocative studies to stress fresh tendencies within the very important box of microbial ecology. Advances in Microbial Ecology is now famous as an immense resource of infor mation and thought either for practising and for potential microbial ecologists. such a lot studies showing in Advances were ready through leaders particularly parts keep on with ing invites issued by way of the Editorial Board. people are inspired, even though, to put up outlines of unsolicited contributions to any member of the Editorial Board for attention for e-book in Advances. With the e-book of quantity 12 of Advances in Microbial Ecology there'll be a transformation of Editor and the complete Editorial Board. the present Editor needs to take this chance to thank the current Editorial Board, Ron Atlas, Bo Barker J~rgensen, and Gwyn Jones, in addition to prior contributors of the Board, for his or her assistance and inspire ment through the years. the hot Editor of Advances in Microbial Ecology can be Gwyn Jones, with Bernhard Schink, Warwick F. Vincent, and David M. Ward as contributors of the Editorial Board. The outgoing Board want the recent Board each luck in continu ing the traditions proven via Martin Alexander, the founding Editor of Advances in Microbial Ecology. the themes featured in quantity 12 of Advances comprise a few on the topic of the meta bolic actions of micro organism; specifically, bioremediation of oil spills, via R. M. Atlas and R.
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Extra resources for Advances in Microbial Ecology
One example is the use of resorufin as an Eh probe to determine the redox potential of bacterial cells. Resorufin is colorless and nonfluorescent in its reduced state but fluoresces red when it becomes oxidized. It is nontoxic and widely used as a redox indicator in microbiological culture media. When it penetrates bacterial cells, it provides an indication of the metabolic state for facultatively anaerobic organisms. In actively metabolizing cells, growing under aerobic but oxygen-limited conditions, the cytoplasm becomes reducing and resorufin is nonfluorescent.
UV lasers are available but prohibitively expensive. The selection of lasers is most critical in dual-labeling experiments when the requirement for narrow bandpass filters greatly decreases the amount of laser light available unless the laser selected has a major excitation peak at both of the desired excitation wavelengths. Using the BioRad MRC 600 microscope, we and others (Harris, 1991) have found that FITC fades. , Chicago Ill. 60614, USA). We have found that RITC is less susceptible and use it as an alternative.
By subtracting the light areas from the corresponding dark areas, calculations of growth rate can be corrected for cell movement. In the analysis of 3-D images the same principle would apply but the light and dark areas would be replaced by light and dark volumes. However, difference imaging has not yet been applied to 3-D volumetric images. 3. Visualization of Microbial Activity and Viability using Fluorescent Molecular Probes Fluorescent molecular probes have also been used to image bacterial metabolic activity.
Advances in Microbial Ecology by Douglas E. Caldwell, Darren R. Korber, John R. Lawrence (auth.), K. C. Marshall (eds.)